DESTINA featuring at Virtual Symposium On Ultrasensitive Immunoassays: The Power of Single Molecule Counting (SMC™) Technology organised by MilliporeSigma

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On Wednesday 31 March 2021, DESTINA’s Juan Jose Diaz-Mochon will present the First Single Molecule Counting (SMC™) Application with miRNAs by a PCR Free Method. You can register here to watch the presentation https://lnkd.in/eiMknq9 The high specificity of DESTINA technologies has been implemented within the ultrasensitive SMC™ to yield the platform’s first molecular assays. During his presentation, Juan…

Amplification-free profiling of microRNA-122 biomarker in DILI patient serums, using the Luminex MAGPIX system

PUBLICATIONS

Citation: Talanta, 2020, doi: 10.1016/j.talanta.2020.121265 Abstract Dynamic chemical labelling is a single-base specific method to enable detection and quantification of micro-Ribonucleic Acids in biological fluids without extraction and pre-amplification. In this study, dynamic chemical labelling was combined with the Luminex MAGPIX system to profile levels of microRNA-122 biomarker in serum from patients with Drug-Induced Liver Injury.

Direct Detection of miR-122 in Hepatotoxicity Using Dynamic Chemical Labeling Overcomes Stability and isomiR Challenges

PUBLICATIONS

Citation: Analytical Chemistry, 2020, doi: 10.1021/acs.analchem.9b05449 Abstract Circulating microRNAs are biomarkers reported to be stable and translational across species. MicroRNA-122 (miR-122) is a hepatocyte-specific microRNA biomarker for drug-induced liver injury (DILI). We developed a single molecule, dynamic chemical labeling (DCL) assay to directly detect miR-122 in blood. The DCL assay specifically measured miR-122 directly from 10…

PCR-free and chemistry-based technology for miR-21 rapid detection directly from tumour cells

PUBLICATIONS

Citation: Talanta, 2019, 51-56 Abstract miRNAs are well known for being implicated in a myriad of biological situations, including those related to serious diseases. Amongst miRNAs, miRNA-21 has the spotlight as it is reported to be up-regulated in multiple severe pathological conditions, being its quantification a key point in medicine. To date, most of the techniques for miRNA quantification…

New Platform for the Direct Profiling of microRNAs in Biofluids

PUBLICATIONS

Citation: Anal. Chem., 2019, 91 (9), 5874–5880 Abstract Circulating microRNAs have been identified as potential biomarkers for early detection, prognosis, and prediction of several diseases. Their use in clinical diagnostics has been limited by the lack of suitable detection techniques. Most of the current technologies suffer from requiring complex protocols, not yet able to deliver robust and cost-effective…

A PCR-free technology to detect and quantify microRNAs directly from human plasma

PUBLICATIONS

Citation: Analyst, 2018, 143, 5676-5682 Abstract A novel sensitive, specific and rapid method for the detection and quantification of microRNAs without requiring extraction from their biological sources is now available using a novel chemical based, PCR-free technology for nucleic acid testing. In this study, we both demonstrate how this method can be used to profile miR-451a,…

Single Molecule MicroRNA Technology Proves Useful for Liver Toxicity Detection

PRESS RELEASES

Breakthrough Research Published in PLOS ONE Demonstrates Quantitative, PCR-Free Detection of MicroRNA LEXINGTON, Mass. and EDINBURGH, UK—July 5, 2017— Quanterix Corporation, a company digitizing biomarker analysis with the goal of advancing the science of precision health, and DestiNA Genomics Ltd, a manufacturer of patented reagents for nucleic acid detection, today announced that PLOS ONE has…

Polymerase-free measurement of microRNA-122 with single base specificity using single molecule arrays: Detection of drug-induced liver injury

PUBLICATIONS

Citation: PLOS One, 2017, doi: journal.pone.0179669 Abstract We have developed a single probe method for detecting microRNA from human serum using single molecule arrays, with sequence specificity down to a single base, and without the use of amplification by polymerases. An abasic peptide nucleic acid (PNA) probe—containing a reactive amine instead of a nucleotide at a specific position…

Novel bead-based platform for direct detection of unlabelled nucleic acids through Single Nucleobase Labelling

PUBLICATIONS

Citation:  Talanta, 2016, 161, 489-496 Abstract Over the last decade, circulating microRNAs have received attention as diagnostic and prognostic biomarkers. In particular, microRNA122 has been demonstrated to be an early and more sensitive indicator of drug-induced liver injury than the widely used biomarkers such as alanine aminotransferase and aspartate aminotransferase. Recently, microRNA122 has been used in vitro to assess the cellular toxicity of…