Evaluation of diagnostic and prognostic candidate biomarkers in drug-induced liver injury vs. other forms of acute liver damage

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Citation: Br J Clin Pharmacol. 2023; 89:2497–2507 DOI:10.1111/bcp.15724 Abstract Aims Detection and characterization of idiosyncratic drug-induced liver injury (DILI) currently rely on standard liver tests, which are suboptimal in terms of specificity, sensitivity and prognosis. Therefore, DILI diagnosis can be delayed, with important consequences for the patient. In this study, we aimed to evaluate the…

MAGPIX and FLEXMAP 3D Luminex platforms for direct detection of miR-122-5p through dynamic chemical labelling

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Citation: Analyst, 2023, Advance Article DOI: 10.1039/D3AN01250F Abstract MicroRNAs (miRs) have emerged as promising biomarkers for diagnosing and predicting the prognosis of liver injury. This study aimed to compare the performance of two Luminex platforms, MAGPIX and FLEXMAP 3D, utilizing the innovative Dynamic Chemical Labelling (DCL) technology for direct detection and analysis of miR-122-5p in…

Amplification-free profiling of microRNA-122 biomarker in DILI patient serums, using the Luminex MAGPIX system

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Citation: Talanta, 2020, doi: 10.1016/j.talanta.2020.121265 Abstract Dynamic chemical labelling is a single-base specific method to enable detection and quantification of micro-Ribonucleic Acids in biological fluids without extraction and pre-amplification. In this study, dynamic chemical labelling was combined with the Luminex MAGPIX system to profile levels of microRNA-122 biomarker in serum from patients with Drug-Induced Liver Injury.

Direct Detection of miR-122 in Hepatotoxicity Using Dynamic Chemical Labeling Overcomes Stability and isomiR Challenges

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Citation: Analytical Chemistry, 2020, doi: 10.1021/acs.analchem.9b05449 Abstract Circulating microRNAs are biomarkers reported to be stable and translational across species. MicroRNA-122 (miR-122) is a hepatocyte-specific microRNA biomarker for drug-induced liver injury (DILI). We developed a single molecule, dynamic chemical labeling (DCL) assay to directly detect miR-122 in blood. The DCL assay specifically measured miR-122 directly from 10…

Smartphone-Based Diagnosis of Parasitic Infections With Colorimetric Assays in Centrifuge Tubes

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Citation: IEEE Access, 2019, 7, 185677 – 185686 DOI:10.1109/ACCESS.2019.2961230 Abstract A smartphone-based platform for the diagnosis of parasitic infections has been developed, tested and validated. The system is capable of making automatic and accurate analysis of millimetric colorimetric arrays in centrifuge collection tubes, which are well established tools used in clinical analysis. To that end, an Android-based…

miR-122 direct detection in human serum by time-gated fluorescence imaging

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Citation: Chem. Commun., 2019, 55, 14958-14961 Abstract A simple method for direct detection of microRNAs (miRs) in human serum without the use of polymerase amplification is presented, achieving low miR-122 concentrations and importantly, discerning effectively single-base sequence mutations. The method is based on the capture of target miRs with synthetic peptide nucleic acid oligomers, dynamic chemical labelling,…

PCR-free and chemistry-based technology for miR-21 rapid detection directly from tumour cells

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Citation: Talanta, 2019, 51-56 Abstract miRNAs are well known for being implicated in a myriad of biological situations, including those related to serious diseases. Amongst miRNAs, miRNA-21 has the spotlight as it is reported to be up-regulated in multiple severe pathological conditions, being its quantification a key point in medicine. To date, most of the techniques for miRNA quantification…

New Platform for the Direct Profiling of microRNAs in Biofluids

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Citation: Anal. Chem., 2019, 91 (9), 5874–5880 Abstract Circulating microRNAs have been identified as potential biomarkers for early detection, prognosis, and prediction of several diseases. Their use in clinical diagnostics has been limited by the lack of suitable detection techniques. Most of the current technologies suffer from requiring complex protocols, not yet able to deliver robust and cost-effective…

A colorimetric strategy based on dynamic chemistry for direct detection of Trypanosomatid species

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Citation: Scientific Reports, 2019, 9, Article number: 3696 Abstract Leishmaniasis and Chagas disease are endemic in many countries, and re-emerging in the developed countries. A rapid and accurate diagnosis is important for early treatment for reducing the duration of infection as well as for preventing further potential health complications. In this work, we have developed a novel colorimetric molecular assay…

A PCR-free technology to detect and quantify microRNAs directly from human plasma

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Citation: Analyst, 2018, 143, 5676-5682 Abstract A novel sensitive, specific and rapid method for the detection and quantification of microRNAs without requiring extraction from their biological sources is now available using a novel chemical based, PCR-free technology for nucleic acid testing. In this study, we both demonstrate how this method can be used to profile miR-451a,…