Identification of Trypanosomatids by detecting Single Nucleotide Fingerprints using DNA analysis by dynamic chemistry with MALDI-ToF

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Citation: Talanta, 2018, 299-307 Protozoan parasites of the Trypanosomatidae family can cause devastating diseases in humans and animals, such as Human African Trypanosomiasis or Sleeping Sickness, Chagas disease and Leishmaniasis. Currently, there are molecular assays for detecting parasitic infections and their post-treatment monitoring based on nucleic acid amplification, but there are still certain limitations which limit the…

Novel molecular assay based on ‘Single Nucleotide Fingerprints’ (SNF) for the diagnosis and monitoring of Trypanosomatids.

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Novel molecular assay based on ‘Single Nucleotide Fingerprints’ (SNF) for the diagnosis and monitoring of Trypanosomatids. EDINBURGH, UK and GRANADA, Spain—July 21, 2017— Researchers at the Centre for Genomics and Oncological Research (GENYO) located in Granada, Spain, in collaboration with DestiNA have developed a novel assay for the diagnosis and monitoring of Trypanosomatids. The method…

Dynamic chemistry for enzyme-free allele discrimination in genotyping by MALDI-TOF mass spectrometry

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Abstract Single nucleotide polymorphisms (SNPs) and insertions/deletions (indels) constitute important sources of genetic variation which provide insight into disease origins and differences in drug responses. The analysis of such genetic variation relies upon the generation of allele-specific products, typically by enzymatic extension or alternatively by the hybridization of DNA probes. MALDI-TOF mass spectrometry (MS) has been widely used as a read-out…

DNA Analysis by Dynamic Chemistry

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Citation: Angewandte Chemie International Edition, 2010, 49, 1809–1812. Abstract Finding flaws: An enzyme‐free method of DNA analysis raises the possibility of analyzing single‐nucleotide polymorphism, indel, and abasic sites using mass spectrometry as a readout tool. The methodology is suitable for the dual analysis of heterozygous samples.