Smartphone-Based Diagnosis of Parasitic Infections With Colorimetric Assays in Centrifuge Tubes

PUBLICATIONS

Citation: IEEE Access, 2019, 7, 185677 – 185686 DOI:10.1109/ACCESS.2019.2961230 Abstract A smartphone-based platform for the diagnosis of parasitic infections has been developed, tested and validated. The system is capable of making automatic and accurate analysis of millimetric colorimetric arrays in centrifuge collection tubes, which are well established tools used in clinical analysis. To that end, an Android-based…

miR-122 direct detection in human serum by time-gated fluorescence imaging

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Citation: Chem. Commun., 2019, 55, 14958-14961 Abstract A simple method for direct detection of microRNAs (miRs) in human serum without the use of polymerase amplification is presented, achieving low miR-122 concentrations and importantly, discerning effectively single-base sequence mutations. The method is based on the capture of target miRs with synthetic peptide nucleic acid oligomers, dynamic chemical labelling,…

Dynamic chemistry for enzyme-free allele discrimination in genotyping by MALDI-TOF mass spectrometry

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Abstract Single nucleotide polymorphisms (SNPs) and insertions/deletions (indels) constitute important sources of genetic variation which provide insight into disease origins and differences in drug responses. The analysis of such genetic variation relies upon the generation of allele-specific products, typically by enzymatic extension or alternatively by the hybridization of DNA probes. MALDI-TOF mass spectrometry (MS) has been widely used as a read-out…

DNA Analysis by Dynamic Chemistry

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Citation: Angewandte Chemie International Edition, 2010, 49, 1809–1812. Abstract Finding flaws: An enzyme‐free method of DNA analysis raises the possibility of analyzing single‐nucleotide polymorphism, indel, and abasic sites using mass spectrometry as a readout tool. The methodology is suitable for the dual analysis of heterozygous samples.