Peptide nucleic acid (PNA) is a DNA mimic with an acyclic, achiral and uncharged pseudo-peptide backbone. The sugar-phosphate groups of DNA are replaced with repeating N-(2-aminoethyl) glycine units.
Affinity and Selectivity
The binding affinity and selectivity of PNA towards DNA, RNA is higher than for the analogous DNA duplexes due to the neutral character of PNA. PNA has better discriminating power with a more effective single base mismatch discrimination than DNA.
PNA oligomers are resistant to enzymatic degradation by proteases and nucleases, which extends their lifetime for applications in vitro and in vivo. PNAs are also stable over a wide pH range and quite stable under high temperature.
PNA is an ideal tool in different areas of life science, such as: a) gene therapy; b) microarray screening; c) SNP (single nucleotide polymorphism) analysis; d) miRNA profiling. PNA synthesis can be carried out by conventional solid phase peptide synthesis.
The DESTINA team has developed an extensive experience in PNA chemistry. Our team will design, synthesize and supply PNAs following strict quality control protocols to meet customer expectations.
γ-PNA is a PNA with a side chain modification at the γ-carbon atom of the backbone. γ-PNA structure modifications provide several advantages vs standard PNA, such as improved solubility, less self-aggregation, more stable PNA-DNA duplex formation, and flexibility for multi labelling and other functionalization.
- C3-NH2, C4-NH2, C6-NH2, C12-NH2
- Polyethylene Glycol linker
γ Backbone modification
- Glutamic acid
- Short peptide-conjugation: Linear synthesis on resin
- Long peptide-conjugation: Ligation through thiol/maleimide or disulfide linkage
Fluorophore and/or Tag
- Cyanine dye
- FAM, FITC
- TAMRA (TMR)
- ATTO dye
- Others …
Minimum Order Quantity (guaranteed final yield):
- 25 nmoles for labeled PNAs
- 50 nmoles for unlabeled PNAs
- Standard Purity: >90% (higher purity is available on request).