The Power Behind PCR-Free Diagnostics

DGL-Tech™ is our proprietary chemistry for the direct, rapid, and highly specific detection of nucleic acids (like miRNA) on standard immunoassay platforms.

DGL-Tech™: The Core Chemistry

DGL-Tech™ for nucleic acid analysis combines patented SMART-Bases with unique peptide nucleic acid (PNA) capture probes with ‘blank’ positions (DGL-Probes).

The revolutionary DGL-Tech™ works thanks to Watson-Crick base pairing (C-G/A-T) that enables the templating of a dynamic reaction on the DGL-Probe.

1. The DGL-Probe hybridises with a complementary Nucleic Acid Target, creating a Chemical Pocket containing the Unknown Nucleotide under interrogation.

2. In the presence of four SMART-Bases (i.e. adenine, thymine guanine and cytosine) the Chemical Pocket creates reversible iminium intermediates between the aldehyde chemical group on the SMART-Bases and the amino group on the blank position.

3. The Unknown Nucleotide stabilises the iminium intermediate formed with the complementary SMART-Base. The most stable iminium intermediate is thus reduced (Chemical Lock-up) and analysed.

ChemiRNA Tech™: The miRNA Application

Profiling of miRNAs is one of the most active areas of research in the field of liquid biopsy. DESTINA offers a new way to interrogate sequences of miRNAs through its unique ChemiRNA™ Tech.

ChemiRNA™ Tech is based on DESTINA DGL-Tech™ for the direct detection and quantification of miRNAs in body fluids. It uses a single biotinylated SMART-Base to label the duplex formation with miRNA sequences.

The technology includes DESTINA proprietary Stabiltech buffer that allows the liberation and stabilisation of RNAs in body fluids, and at the same time removes the need for refrigerated handling up to the analysis.

1. The DGL-Probe captures the complementary miRNA sequence, forming the Chemical Pocket. When the target miRNA hybridisation is complete, the biotinylated SMART-Base is covalently attached to the backbone of the DGL-Probe (Chemical Lock-up).

2. The duplex is read out using a Reporter Molecule that recognise the biotin tag.

Get Started with DGL-Tech™

For Researchers (Pharma/Biotech)

See this technology in action. Test our validated, ready-to-use LiverAce™ kits for DILI safety assessment today.

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For Partners (CROs/Diagnostics)

License DGL-Tech™ to create your own novel, PCR-Free assays. Gain a competitive advantage in the biomarker market.

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